Please enable JavaScript or talk to your local administrator to get JavaScript enabled.
Skip filters
Skip facets
Funder
  • 13

  • 8

  • 3

  • 2

  • 2

  • 2

  • 1

  • 1

Funder Group
Research Organization
FOR (ANZSRC) Category
RCDC Category
Researcher
City
State
Country
Country of Funder
Start Year
Active Year
Similar Projects for

Project

[Molecular mechanism of bladder cancer invasion and metastasis focusing on invadopodia, exosome, and hyaluronidase]

Original in Japanese: Molecular mechanism of bladder cancer invasion and metastasis focusing on invadopodia, exosome, and hyaluronidase

Funder: Japan Society for the Promotion of Science

Funding period
JPY 4.2 M
USD 38 K
Funding amount
Abstract
[Recently, the role of exosomes in the invasion and metastasis of cancer cells has been drawing attention. The scaffold (invadopodia: Invadopodia) extended by bladder cancer cells is essential for invasion and metastasis, and the invasion process secretes exosomes. There are mRNAs, miRNAs, enzymes, etc. in exosomes, but there are still many unclear points. Exosomes may support the invasion and metastasis of cancer by preparing an environment (pre-metastatic niche) that allows easy survival. Since the exosome contains an enzyme that decomposes hyaluronan, which is a cell barrier, it is likely to be important for the transmission of exosome contents. However, since there are no studies investigating cancer invasion and metastasis in terms of exosomes and hyaluronidase, this study focuses on hyaluronidase in exosomes secreted by the infiltrates of bladder cancer cell lines, and determines the mechanism of bladder cancer invasion and metastasis. Elucidate. In this research, the following research was conducted in FY2018. (1) We established a method for preparing relatively large amounts of exosomes by ultracentrifugation from three types of urothelial cancer cell lines, KK47, RT-4, and YTS-1. The particle size and particle size of the prepared exosomes were measured using a nanoparticle multi-analyzer qNano. In addition, the expression of exosome markers CD9 and TSG10 was detected by Western blotting. From the above two data, it was confirmed that exosomes could be prepared. Furthermore, we established a method for fluorescently labeling exosomes with the fluorescent dye PKH67 and tracking the process of intracellular uptake when incubated with cells. (2) Using the urothelial carcinoma cell line YTS-1, we established the Cortactin KD YTS-1 strain in which the expression of cortactin, which is a protein essential for infiltrating process formation, was suppressed by shRNA. Of the four research projects, we completed the plan ① and established Cortactin KD YTS-1 cells. With the results so far, we are ready for an experiment to examine the role of exosomes listed in ② on the invasion and metastasis of cancer cells.]
Original in Japanese
近年、がん細胞の浸潤や転移におけるエクソソームの役割が注目されている。膀胱癌細胞が伸ばす足場(浸潤突起:Invadopodia)が浸潤・転移に必須であり、浸潤突起はエキソソームを分泌する。エキソソームの中にはmRNA、miRNA、酵素などが存在するが、未だ不明な点が多い。エキソソームはあらかじめ生存しやすい環境(前転移ニッチ)を整えて癌の浸潤・転移を補助している可能性がある。エキソソーム内には細胞バリアであるヒアルロン酸を分解する酵素がに含まれていることから、エキソソーム内容の伝達に重要である可能性が高い。しかしエキソソームとヒアルロニダーゼの点から癌の浸潤・転移を検討している研究はないため、本研究では膀胱癌細胞株の浸潤突起が分泌するエキソソーム内のヒアルロニダーゼに着目し膀胱癌浸潤・転移機序の解明を行う。本研究ではH30年度に以下の研究を行った。①3種の尿路上皮癌細胞株、KK47、RT-4、YTS-1から、超遠心により比較的大量にエキソソームを調製する方法を確立した。調製されたエキソソームの粒度・粒径を、ナノ粒子マルチアナライザーqNanoを使用して測定した。また、ウェスタン・ブロッティングによって、エキソソーム・マーカーであるCD9、TSG10の発現を検出した。上記2つのデータから確かにエキソソームの調製ができたことを確認した。さらに、蛍光色素PKH67を用いてエキソソームを蛍光標識し、細胞内とインキュベートした際に細胞内に取り込まれる過程を追跡する方法を確立した。②尿路上皮癌細胞株YTS-1を用いて、この浸潤突起形成に必須のタンパク質であるコータクチン(Cortactin)の発現を、shRNAにより抑制したCortactin KD YTS-1株を樹立した。本研究計画4つのうち、①の計画を完遂し、さらに、Cortactin KD YTS-1細胞を樹立した。ここまでの結果で、②で掲げたエキソソームが癌細胞の浸潤・転移に果たす役割を検討する実験の準備が整った。

 
32
Projects
USD 12.2 M
Aggregated funding amount
USD 469 K
Average funding amount
Project list item
Defining NRF2 induced tumor invasion in bladder cancer

Bladder Cancer Advocacy Network to Yuki Kita, William Youngkwan Kim, Bernard Weissman

USD 50,000
2020 - 2021
Project list item
The role of a new molecular driver in bladder cancer

National Cancer Institute to DALEY MORERA

USD 90,032
2018 - 2021
Project list item
Modeling bladder cancer metastasis using human patient-derived tumor organoids

Bladder Cancer Advocacy Network to Michael M Shen

USD 300,000
2018 - 2020
Project list item
Role of B-arrestins in bladder cancer progression and response to chemotherapy

United States Department of Veterans Affairs to BAL L LOKESHWAR

 
2018 - 2022
Project list item
[Functional analyses of oncogenic microRNA by genome editing and development of innovative genome-based drug discovery for bladder cancer.] - Original in Japanese

Japan Society for the Promotion of Science to Hideki ENOKIDA, Masayuki NAKAGAWA, Hirofumi YOSHINO

USD 154,520
2017 - 2020
Project list item
The Role of EZH2 in Non-Muscle Invasive Bladder Cancer

United States Department of Veterans Affairs to JOSHUA JAMES MEEKS

 
2016 - 2020
Project list item
To develop a new therapeutic method targeting AKR1C1 for urothelial carcinoma micrometastasis

Japan Society for the Promotion of Science to Ryuji Matsumoto

USD 22,319
2016 - 2018
Project list item
Analysis of Heparanase for invasion abilities of bladder cancer cells

Japan Society for the Promotion of Science to Yoshihiro Tatsumi

USD 32,892
2016 - 2018
Project list item
Investigation on usefulness of Fes / Fer as new therapeutic target and predictive factor in urothelial cancer

Japan Society for the Promotion of Science to Kosuke TAKEHARA, Yasuyoshi MIYATA, Yasushi MOCHIZUKI

USD 42,591
2015 - 2018
Project list item
Analysis of cancer-specific urinary exosomal miRNA derived from urothelial carcinoma

Japan Society for the Promotion of Science to Kyosuke Matsuzaki

USD 34,348
2015 - 2017
Project list item
Elucidation of novel carcinogenesis / development pathway of bladder cancer by using metabolism-related omics analysis

Japan Society for the Promotion of Science to Naoki TERADA, Yoshiyuki MATSUI, Takashi Kobayashi

USD 45,237
2014 - 2017
Project list item
Exosomes: A novel mechanism for bladder cancer tumorigenesis and progression

National Cancer Institute to YI-FEN LEE

USD 1,812,082
2014 - 2019
Project list item
Specific inhibition of Notch2 as a novel therapy for invasive bladder cancer

Canadian Institutes of Health Research to Tetsutaro Hayashi

USD 969
2013 - 2013
Project list item
NVP-BEZ235 Therapy for Bladder Cancer and Prevention of Tumor Invasion and Metastasis

Japan Society for the Promotion of Science to Masashi MATSUSHIMA, Mototsugu OYA, Akira MIYAJIMA, Eiji KIKUCHI

USD 38,694
2013 - 2015
Project list item
Progranulin signaling in bladder cancer

National Cancer Institute to RENATO V. IOZZO, ANDREA MORRIONE

USD 1,579,179
2012 - 2018
Project list item
Protein, mRNA and miRNAs profiling in urothelial bladder carcinoma

São Paulo Research Foundation to Fernando Augusto Soares

 
2012 - 2014
Project list item
Bladder Cancer Program

National Cancer Institute to ANDREA APOLO

USD 3,889,686
2010 - 2019
Project list item
The Role of Thromboxane A2 (TP) Receptor Beta in Bladder Cancer

National Cancer Institute to OMAR MOUSSA

USD 1,484,957
2009 - 2015
load more...